Naturally occurring aesculetin coumarin exerts antiproliferative effects in gastric cancer cells mediated via apoptotic cell death, cell cycle arrest and targeting PI3K/AKT/M-TOR signalling pathway

Aesculetin is an active member of coumarins that has been reported to possess significant medicinal and biological importance. It also shown with potential anticancer activity against different human cancers including breast, lung hepatocellular carcinoma. Therefore, the current investigation was undertaken examine effects aesculetin gastric cancer. MTT assay performed check cellular viability clonogenic executed assess effect on colony formation capacity SGC-7901 cancer cells. Apoptosis analysed by AO/EB staining annexin V-FITC/PI assays. Cell cycle phases were monitored using flowcytometry western blotting used detect PI3K/AKT/M-TOR signalling pathway. Results indicated not only reduced proliferation in time-dependent manner but dose-dependent as well. Clonogenic tendency cells retarded significantly aesculetin. The antiproliferative may arbitrate via apoptosis. Further, flow cytometric analysis revealed G2/M-phase amplified number increasing doses. Indicating blocking cell at G2/M-phase. Finally, suggested pathway Taking altogether, could induce growth inhibitory Moreover, apoptotic death, arrest block